Transgenic/Knock-out Mouse

SERVICE DESCRIPTION

CINJ MEMBER FEES

PER

Generation of Transgenic founders by DNA microinjection

$2200

Construct

Generation of Chimeric mice by embryonic stem cell microinjection

$2000

2-3 cell lines

Gene Targeting in mouse embryonic stem cells

$7500

Construct

Chimera breeding (2 males and 3 litters)

$ 150

Cell line

Mouse embryonic stem cell culture (all materials provided except ESGRO)

$50

Hour

Mouse primary fibroblast preparation

$50

Hour

Embryo Cryopreservation

$1500

Strain

Sperm Cryopreservation

$1000

Strain

Re-derivation by in vitro fertilization

$1000

Strain

Re-derivation by embryo transfer

$1500

Strain

Tail DNA preparation

$1

Tail

Tail DNA preparation & Phenol:Chloroform extraction

$2

Tail

Genotyping by Southern blot (maximum 40 samples)

$300

Blot

Genotyping by PCR (minimum 10 samples)

$1.50

Sample

PCR set up charge (Maximum 3 gradient PCR reaction)

$100

3-reactions

Training - mouse embryonic stem cell culture (all materials provided except ESGRO)

$50

Hour

Training – mouse primary fibroblast culture (all materials provided)

$50

Hour

Mouse primary embryonic fibroblast treated with Mitomycin-C or g-irradiated (10 7 cells)

$100

Vial

Mouse primary embryonic fibroblast (Passage-3/ 10 7 cells)

$100

Vial

Mouse purchase from Commercial vendor. Price will vary depending on strain, sex and vendor.

By Vivarium

Mouse per diem charges – if not transferred after weaning and Males other than F1 (B6xCBA)

$0.152

Mouse/day

Euthanasia upon request

$0.20

Mouse

Carcass disposal

$0.040

Mouse

Enzyme purchase depending on enzyme, vendor and amount

FBS ES qualified/tested

$300

500ml

Core Personnel per diem for Husbandry service

$30

Hour

Liquid nitrogen storage Fees

$120/Year

Line

Mice transfer to-and-from NRB Vivarium

By Vivarium