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Flow Cytometry Home | Services | Equipment | Price List | Location | Contact Us Equipment Coulter Cytomics FC500 flow cytometers: These are user-friendly analytical instruments with one air-cooled argon laser and one air-cooled helium-neon laser that permit simultaneous measurement of five different fluorescent probes. These instruments are used for routine multiparameter analysis of DNA content, cell cycle, immunofluorescence, apoptosis, green fluorescence protein expression, oxidative metabolism and phagocytosis. Two Cytomics FC500 instruments are currently located on the Busch campus, and staffed by Theresa Choi and Joan Dubois. A third Cytomics FC500 is located at CINJ and is staffed by Art Roberts. Schedule the Flow Cytometer at CINJ Beckman-Coulter MoFlo XDP Cell Sorter: The MoFlo allows rapid separation of large numbers of specific cell populations with high purity, recovery and yield. It an extremely stable platform allowing for analysis at rates up to 100,000 events/second and sort speeds of up to 70,000 events/second. In addition, the instrument is capable of simultaneous 4 parameter sorting. The MoFlo is equipped with 3 solid-state air-cooled lasers allowing for excitation wavelengths of 355, 488 and 633 nm and 9 PMTs making it possible to simultaneously analyze 12 different cell properties. The instrument also has a biosafety containment cabinet and an automatic shut-down mode which will permit sorting of transfected human cells. In addition, the MoFlo is equipped with the Cyclone® sorting option for single cell cloning or for sort matrix verification. BD FACSArray Bioanalyzer: This instrument rapidly detects and quantifies concentrations of secreted proteins, proteins in cell lysates, and cell-associated proteins using small sample volumes directly from a 96-well microtiter plate through multiplexed BD Cytometric Bead Arrays (CBAs). The bioanalyzer is for fast and sensitive high-content measurements of cells and proteins in cell biology, immunology, and proteomics. It supports the research in cellular analysis of apoptosis, cytokine and chemokine profiling, and phosphorylation of key signal transduction proteins. It can also be used to assess hybridomas, cell viability, proliferation, and immunotoxicology. Throughput for acquisition of a 96-well plate is estimated at 35 minutes or less for most multiplex bead and cellular assays. The bioanalyzer is equipped with a green (532nm) and a red (635nm) laser to deliver up to six-parameter detection (four fluorescence and two scatter) at one time. State-of-the-art digital signal processing allows up to 15,000 events per second.
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Shared Resources
Functional Genomics Facility (formerly Transcriptional Profiling Facility) Transgenic/Knock-out Mouse Facility Office of Human Research Services Centralized Education and Training Services (CETS) Pharmacokinetic and Pharmacodynamic (PK/PD) Protein Structure Analysis and Modeling Facility (PSAM) Additional Shared Resources
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