Flow Cytometry/Cell Sorting - Equipment
Beckman Coulter Gallios Flow Cytometer (Piscataway): The Gallios flow cytometer is an ultra sensitive high speed bench top flow cytometer with 3 solid state lasers (405, 488, and 638 nm) and 10 fluorescence colors. In addition, because of the high stability and sensitivity of the instrument, very dim and rare populations can be accurately analyzed. Gallios has a customized forward scatter to achieve the most accurate size measurement and optimized electronics for high resolution and fast, accurate, and efficient processing. The instrument can analyze particle sizes from 0.4 µm to 40 µm diameter. The Gallios is an upgraded flow cytometer when compared to the existing Beckman Coulter FC500 flow cytometer in the Core facility. Data acquired by Gallios can be analyzed by Kaluza analysis software, which is available for users to analyze on their own computer. In addition, data collected on the FC500 instruments can also be analyzed on the new Kaluza software.
Beckman-Coulter Cytomics FC500 Flow Cytometers (Piscataway/New Brunswick): These are user-friendly analytical instruments with a blue (488 nm) argon laser and a red (640 nm) helium-neon laser that permit simultaneous measurement of five different fluorescent probes. These instruments are used for routine multiparameter analysis of DNA content, cell cycle, immunofluorescence, apoptosis, green fluorescence protein expression, oxidative metabolism and phagocytosis. Two Cytomics FC500 instruments are located at the Environmental and Occupational Health Sciences Institute in Piscataway and a third is located at the Rutgers Cancer Institute of New Jersey in New Brunswick
Beckman-Coulter MoFlo XDP Cell Sorter (Piscataway): The MoFlo allows rapid separation of large numbers of specific cell populations with high purity, recovery and yield. It is an extremely stable platform allowing for analysis at rates up to 100,000 events/second and sort speeds of up to 70,000 events/second. In addition, the instrument is capable of simultaneous 4-parameter sorting. The MoFlo is equipped with 3 solid-state air-cooled lasers allowing for excitation wavelengths of 355, 488 and 633 nm and 9 PMTs making it possible to simultaneously analyze 12 different cell properties. The instrument is enclosed in a class II-certified biohazard containment cabinet with an automatic shut-down mode which will permit sorting of human and primate cells. In addition, the MoFlo is equipped with the Cyclone® sorting option for single cell cloning or for sort matrix verification. This instrument is located in the Piscataway facility.
BD Biosciences FACSArray Bioanalyzer (Piscataway): This instrument rapidly detects and quantifies concentrations of secreted proteins, proteins in cell lysates, and cell-associated proteins using small sample volumes directly from a 96-well microtiter plate through multiplexed BD Cytometric Bead Arrays (CBAs). The bioanalyzer is for fast and sensitive high-content measurements of cells and proteins in cell biology, immunology, and proteomics. It supports the research in cellular analysis of apoptosis, cytokine and chemokine profiling, and phosphorylation of key signal transduction proteins. It can also be used to assess hybridomas, cell viability, proliferation, and immunotoxicology. Throughput for acquisition of a 96-well plate is estimated at 35 minutes or less for most multiplex bead and cellular assays. The bioanalyzer is equipped with a green (532nm) and a red (635nm) laser to deliver up to six-parameter detection (four fluorescence and two scatter) at one time. State-of-the-art digital signal processing allows up to 15,000 events per second.
BD Biosciences Influx High Speed Cell Sorter (New Brunswick): This open-architecture, high-speed cell sorter allows rapid separation of large numbers of specific cell populations with high purity, recovery, and yield. Using up to 4 different lasers, cell sorting is based on acquisition of up to 14 parameters, including 12 fluorescence parameters. These solid-state lasers provide the ability to detect an array of fluorophores. The Influx can sort cells aseptically at rates up to 20,000 cells per second, or higher (upper limit of 200,000 events/sec), depending on the cell size and type. In addition, four different subpopulations can be collected simultaneously. The optical detection system has interchangeable filters for custom applications, allowing optimization to the needs of researchers. Cells can be sorted into various configurations of collection vessels, including most automated single-cell sorting into almost any type of plate configuration, including custom layouts. The Influx is enclosed in a class II-certified biohazard containment cabinet allowing for safe sorting of human and primate cells. The system can be easily sterilized for aseptic sorting. Additionally, for specialty applications, the entire sample handling system can be removed and autoclaved or exchanged for a new gamma irradiated unit.
BD LSRII w/ HTS Analyzer (Princeton): This is a user friendly five-laser fixed alignment bench top instrument with 355, 405, 488, 561 and 635 nm laser excitation sources. It is capable of up to 24-color acquisition and is equipped with HTS 96-well and 384-well automated microsamplers.
iCyt Reflection Cell Sorter (Princeton): This is a new state-of-the-art cell sorter (no use during 12-month use reporting period) that provides users with two independent sort modules capable of simultaneously separating samples with no crossover. Each sort module has five excitation lines concurrently available (355, 405, 488, 561 and 635 nm). The instrument is capable of at least 16 parameter acquisition and can sort samples into 96 well plates or four tubes. The cytometer is contained within a Baker Biological Safety Cabinet, increasing sterility of sorts and permitting safe sorting of human transfected cells.
BD FACSVantage w/ DiVa (Princeton): This is a three laser cell sorting system with advanced digital acquisition (DiVa) technology. It features tunable laser excitation sources (ultraviolet, 488, 568 and 647nm lines are most commonly utilized). It is capable of up to 10 parameter (8-color) acquisition and performs cell sorting into multi-well plates or four tubes.
Union Biometrica COPAS BioSort (Princeton): This large particle cytometer is capable of analyzing, sorting and dispensing cells and organisms 40-175mm in size. It is optimal for use with all stages of C. elegans. It features multiple laser excitation sources: 488 and 514nm with five parameter (3-color) acquisition; a ReFlx system for sampling directly from 96-well plates; and a Profiler II option that allows for the spatial mapping of fluorescence signals along the length of the organism.
BD FACSort (Princeton): This instrument was available to trained users well into the 12-month use reporting period, but has been phased out. This analyzer features dual laser fixed alignment in a benchtop system, with 488 and 635nm laser excitation sources and simultaneous 4-color acquisition capability.